dos.5. Ca dos+ imaging into the neonatal OHCs This new rodent Ocm gene (series in the Shape S01) is actually cloned to the pcDNA3.1CFP and you can pEGFP-N1 Banner (Addgene #60360). Next, EGFP-Banner is actually eliminated using BamHI (NEB) and NotI (NEB) and you will substituted for this new mCherry sequence (gene stop of mCherry […]
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